High Performance Liquid Chromatography

HPLC works in a manner very similar to gas chromatography. As the sample passes through the chromatography column, an equilibrium is established in which the molecules go from being bound to the column and passing through it in the "mobile phase". This equilibrium will be dependent on the relative attraction of the molecules to the column. Thus, we can separate our sample into its various components and determine how much of each is present.

Agilent 1100 Series HPLC with quaternary pump, degasser, autosampler, variable wavelength detector, refractive index detector and Chemstation Data System

Shimadzu HPLC system with variable wavelength detector

Page last modified May 11, 2011