Confocal Training Videos

  Training Videos Available on Panopto 

Confocal Training Materials

Capturing ROIs

• Using Autofocus
• Using Binary Layers
• Using Object Classifiers
• Binary Masking
• Using Pixel Classifier/Area Fraction
• Region of Interest
• Using References
• Scanning Large Images

Converting Formats

• Creating and Viewing AVI Files
• Preparing TIFFs for Microsoft Word
• Using MovieMaker
• Importing Statistics into Microsoft Excel

Photobleaching

• FRAP Experiments on Nikon A1
• Sequential FRAP on Nikon A1
• Sequential Photoactivation on Nikon A1

Data Analysis

• Blind Unmixing Images
• Pearsons and Manders Image Pro
• Setting up Fura-2 Ratios
• HDR Imaging
• Grid to ND Setup
• ND Processing Tools Tutorial
• Counting Objects
• Spectral Analysis

Setup

• Calibration
• Running Well Plate Macros
• Manual Microscope Setup
• Nikon A1 Startup and Shutdown Procedures

IT Software Documents

• Installing NIS-Elements 4.00
• Configuring Multi-User Layouts
• Using Dual Monitors
• Installing the Lumenera Drivers
• Uninstalling NIS-Elements
• Reporting NIS Software Issues
• Troubleshooting Software Issues

User Guides

• NIS-Elements AR User Guide
• NIS-Elements C User Guide (Part 1)
• NIS-Elements C User Guide (Part 2)
• Restoring Layouts in NIS-Elements